1. Place the trephine’s outer shell on the recipient cornea.
2. Activate the vacuum to secure the cornea in place.
3. Gently rotate the internal blade using calibrated markers.
4. Monitor depth using the non-reflective pin – 0.063 mm per quarter turn.
5. Continue until the diseased corneal button is completely excised.
6. Carefully remove the trephine and proceed with graft placement.

> Note: Must be handled by qualified ophthalmic surgeons trained in corneal transplant procedures